Spinning Disk Confocal


See Brighter, See Faster, See More


High-sectioning and High-speed in 25mm Large Field of View makes X-Light V3 capable for the modern fluorescence microscopy applications.  

The first confocal unit which allows dual camera imaging at the full field of view of 25 mm on both cameras.

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Large image of a day 50 human
cerebral organoid

Ultra Large Field of View

Micro-lenses make the illumination field highly homogeneous on large fields
of view (up to 25mm). With large FOV and high-speed imaging
(15,000 rpm), quantitative imaging on large samples becomes more
simple.

Flexible switching between different illumination sizes to fit your application.
Achieving higher power density for higher speed imaging (1KHz) is
possible by changing to a smaller illumination area.

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Full Compatibility

- Microscope (Nikon Motorized Inverted Microscope Ti2-E, with 25mm FOV)
- Illuminators (Lumencor)
- Detectors (Andor)
- Software (Nikon NIS-Elements)

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Brain organoid derived from human iPSC line (MAP2-AF488, labels microtubules in neuronal dendrites, TBR1-AF750 labels nuclear
transcription factor, and DAPI stains DNA

Dual Camera

Dual camera imaging at the 25mm full field of view on both cameras

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Sealed Spinning Disk Box

Makes the Spinning disk fully isolated and protected from environmental dust and prevents undesired artifacts related to the presence of small dust particles on the Spinning disk surface

*Photos and Videos courtesy of CrestOptics official website

Applications

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High Content Imaging

A study of living cells using microscopy to obtain a better understanding of biological function through the study of cellular dynamics.

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Fluorescence

Fluorescence itself is a form of luminescence that results from matter emitting light of a certain wavelength after absorbing electromagnetic radiation. Fluorescence Imaging help visualize biological processes taking place in a living organism.

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Neurobiology / Optogenetics

Is the study of the nervous system. The scope of neuroscience has broadened over time to include different approaches, such as optogenetics, a biological technique to control the activity of neurons or other cell types with light. This is achieved by the expression of light-sensitive ion channels, pumps, or enzymes specifically in the target cells.

Related Products

Microscope

Nikon Inverted Microscope
Ti2-E
Nikon Confocal Microscope System
AX / AX R
Nikon Super Resolution N-SIM
Nikon Super Resolution N-STORM

Camera

PCO. Fluoresence Lifetime Imaging Cameras
FLIM
PCO. High-Speed Cameras
Dimax
Nikon Scientific Color Cameras
Digital Sight 10
Nikon Scientific Monochrome Cameras
DS-Qi2

Software

Nikon NIS-Elements
High Content Analysis HC
Nikon NIS-Elements C
Nikon NIS-Elements AR

LED Light Source for Fluorescence

Nikon LED Light Source for Fluorescence
D-LEDI
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