Neurobiology / Optogenetics

Is the study of the nervous system. The scope of neuroscience has broadened over time to include different approaches, such as optogenetics, a biological technique to control the activity of neurons or other cell types with light. This is achieved by the expression of light-sensitive ion channels, pumps, or enzymes specifically in the target cells.

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Software

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Application Examples

Neuromuscular Junctions 

High-Definition Imaging of Mouse Neuromuscular Junction Using a Resonant Scanner

The structure of a neuromuscular junction in a mouse captured by high-definition 3D imaging using a resonant scanner.

Keywords: high-speed XY-Z, high resolution, large FOV

High Resolution 3D Imaging of Neuromuscular Junction (NMJ) in a Mouse

Nerve endings adhering to muscle cells. The white arrows indicate the distribution of acetylcholine receptors (red) surrounding the neurons (green)

DAPI: cell nucleus (blue), GFP: neurons (green), Alexa 555: acetylcholine receptor (red), CMDR: cell membrane (gray)
Objective: CFI Plan Apochromat Lambda S 25xC Sil

Large Field-of-view Imaging Using a Resonant Scanner

Entire view of mouse muscle tissue. Using a resonant scanner, 6 images were tiled during Z-stack imaging. Image resolution: 1024 x 1024 pixels. MIP display of captured Z-stack images. Objective: CFI Plan Apochromat Lambda 10X

Microscope Involved

Since a resonant scanner can perform confocal imaging with higher temporal resolution than a Galvano scanner, it is used in many cases to acquire life phenomena occurring at high speeds. In contrast, because the resonant scanner of the new generation AX R confocal microscope system supports up to 2K x 2K acquisition, it can be used for a wide range of purposes, from high-speed imaging to high-resolution imaging. Learn More...

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*Photos courtesy of Nikon official website

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