CrestOptics DeepSIM

Super-Resolution Microscope System


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DeepSIM Introduction

Based on a multi-spot structured illumination system, the DeepSIM is a reliable, simple to use and affordable solution to study cellular structures up to an XY resolution of ~100nm.

Super-resolved optical sectioning, with Z resolution up to ~ 300nm, can be obtained using both high (60X – 100X) and low magnification (20x – 40x) objectives to expand the range of applications to include complex 3D models such as tissues, organoids, spheroids, and small organisms.

Features

High Resolution

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Super-resolved optical sectioning with Z resolution up to ~300nm

3D movie of DeepSIM SR acquisition of mouse brain tissue section showing neurons with dendritic spines (yellow), microglia (magenta), astrocytes (white), and DNA (cyan)

High Variety of Samples

Complex 3D models of tissues, organoids spheroids, etc, can be captured even with 50um thickness.
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3D Volume Views of a region of interest showing bacterial cells

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Human brain organoids showing CTIP2-positive deep layer cortical neurons (green) and pan-neuronal MAP2 marker (red)

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mouse brain sample with GFP-expressing microglia

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Live Cell Imaging

With a temporal resolution of >10 fps, with the Deep SIM, it is possible to follow live-cell dynamics at a cellular and sub-cellular level.

DeepSIM enables the effortless study of live-cell dynamics through a temporal resolution greater than 10fps (1024×1024 px FOV), allowing biological changes to be tracked at cellular and subcellular levels.

High Flexibility

DeepSIM is the first super-resolution module that is compatible with any existing upright or inverted microscope with a camera port. Its high compatibility with different samples enables scientists to access deep data to progress their research.
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Expandable system as add-on module for Spinning Disk Confocal

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Standalone equipment

*Photos and videos courtesy of CrestOptics official website

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