High Resolution
Super-resolved optical sectioning with Z resolution up to ~300nm
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DeepSIM Introduction
Based on a multi-spot structured illumination system, the DeepSIM is a reliable, simple to use and affordable solution to study cellular structures up to an XY resolution of ~100nm.
Super-resolved optical sectioning, with Z resolution up to ~ 300nm, can be obtained using both high (60X – 100X) and low magnification (20x – 40x) objectives to expand the range of applications to include complex 3D models such as tissues, organoids, spheroids, and small organisms.
Super-resolved optical sectioning with Z resolution up to ~300nm
3D movie of DeepSIM SR acquisition of mouse brain tissue section showing neurons with dendritic spines (yellow), microglia (magenta), astrocytes (white), and DNA (cyan)
3D Volume Views of a region of interest showing bacterial cells
Human brain organoids showing CTIP2-positive deep layer cortical neurons (green) and pan-neuronal MAP2 marker (red)
mouse brain sample with GFP-expressing microglia
With a temporal resolution of >10 fps, with the Deep SIM, it is possible to follow live-cell dynamics at a cellular and sub-cellular level.
DeepSIM enables the effortless study of live-cell dynamics through a temporal resolution greater than 10fps (1024×1024 px FOV), allowing biological changes to be tracked at cellular and subcellular levels.
Expandable system as add-on module for Spinning Disk Confocal
Standalone equipment
*Photos and videos courtesy of CrestOptics official website
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