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Mightex Polygon400 Selection Guide

All Mightex Polygon400 series supports or is compatible with Electro-physiology & Optogenetics, TTL input triggering, TTL output for sychronization, analog voltage control option*, arbitary waveforms*, mapping between spatial patterns and LED wavelengths*.

Please visit the Mightex website for details.

SpecificationE-Series G-Series Dense Pixel Polygon400 (DP) Fiber-coupled Polygon400 (DL)
Target ApplicationsOptogenetics
Photoactivation
Photostimulation
Photopatterning
Calcium imaging
Optogenetics
Photoactivation
Photostimulation
Photopatterning
Calcium imaging
Uncaging
Photobleaching
Sectioning and Super Resolution Microscopy
Optogenetics
Photoactivation
Photostimulation
Photopatterning
Calcium imaging
High intensity applications
Large field of view applications
Wavelength RangeVisible/NIR
(optimized for 400~700nm)
Visible/NIR
(optimized for 400~700nm)
Visible/NIR
(optimized for 400~700nm)
Visible/NIR
(optimized for 400~700nm)
Light SourcesBuilt-in LEDs onlyLED, Xenon, Arc lamp,
Halogen lamp, etc
LED, Xenon, Arc lamp,
Halogen lamp, etc
LED, Xenon, Arc lamp,
Halogen lamp, etc
Optical InterfaceN.A.
(with built-in LEDs)
3mm-core lightguide
(with 5mm ferrule)
3mm-core lightguide
(with 5mm ferrule)
3mm-core lightguide
(with 5mm ferrule)
Projection AreaLarge
(e.g. 0.8mm x 1.4mm @10x)
Large
(e.g. 0.8mm x 1.4mm @10x)
Large
(e.g. 0.8mm x 1.4mm @10x)
Large
(e.g. 0.8mm x 1.4mm @10x)
ResolutionHigh
(e.g. 1.6μm @10x, and 0.4μm @40x)
High
(e.g. 1.6μm @10x, and 0.4μm @40x)
High
(e.g. 1.6μm @10x, and 0.4μm @40x)
High
(e.g. 1.6μm @10x, and 0.4μm @40x)
Microscope Coupling OptionsInfinity Path
C-mount (camera port)
Infinity Path
C-mount (camera port)
Infinity Path
C-mount (camera port)
Infinity Path
C-mount (camera port)
Software CompatibilityPolyScan,
MicroManager,
NeuroPG,
NIS Elements (pending)
PolyScan,
MicroManager,
NeuroPG,
NIS Elements
PolyScan,
MicroManager,
NeuroPG,
NIS Elements
PolyScan,
MicroManager,
NeuroPG,
NIS Elements

*Has hardware requirements. Please contact sales representitive for more details.

Which Mightex Polygon 400 is for you?

The E-Series Polygon400 has LEDs (up to three) built into the unit that range from 400-700nm and these LEDs are controlled by advanced external LED drivers. The Polygon400 E-Series is one of the most straightforward and cost effective solution for applications requiring patterned illumination, such as in vitro optogenetics. Polygon E-Series can be considered when only a limited number of wavelengths are required by the researcher. 

The G-Series Polygon400 uses any external light that can accept a lightguide (3mm core with ferrule diameter of 5mm and length of 15mm or greater), such as LEDs, arc lamps, and halogen lamps, to provide patterned illumination capabilities. These light sources are driven by external drivers for advanced control, and the use of external light sources provides high output power on the sample, for applications such as uncaging, large field of stimulation optogenetics, and in vivo applications. The Polygon400 G-Series provides greater flexibility for present and future applications using patterned illumination because there is no limit to number of wavelengths that can be used by the researcher. 

Mightex Polygon400 DP is designed for super-resolution and structured-illumination(SI) applications where smaller pixels are required. Due to its high UV(down to 350nm) throughput Polygon400 DP is also an excellent choice for uncaging, photoactivation and photobleaching.

In Polygon DP the projected DMD array is de-magnified compared to the standard Polygon400. The resulted dense pixels with sub-diffraction-limited dimensions can produce dense grating patterns with precise control in pitch and phase, which are critical for SI-based super-resolution microscopy. 

Mightex Fiber-coupled Polygon400 is designed for high intensity and/or large field of view applications such as in-vivo whole brain optogenetic stimulation. The fiber-coupled Polygon400 takes a high power fiber-coupled light source (e.g. laser, LED, Hg Arc lamp, etc) as input to produce high intensity patterned illumnation on the sample. Due to its high power throughput, researchers are able to work with a low-magnification or even a macroscope objective to cover a large stimnulation area, while maintaining sufficient intensity for light stimulation.

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