PCO_FLIM

pco.flim

pco.flim

Large range of lifetimes - Measure lifetimes in the range of 100 ps – 100 µs
High-speed imaging - pco.flim reads out 45 double frames/s at a max with its 1008 x 1008 pixels resolution
Easy to apply - compatible with Nikon's NIS-Elements, with the use of C-mount and USB 3.0 interface

Application:

Luminescence Lifetime Imaging of Chemical Sensors e.g. Oxygen, pH
Fluorescence-guided Neurosurgery - Tumor tissue is clearly distinguished from the shorter autofluorescence of the normal tissue

and so on......

"We believe the pco.flim system will be able to do

this and has the potential to be broadly applied in chemical

imaging."

Reference:

Erkkilä, M. T., Bauer, B., Hecker‐Denschlag, N., Madera Medina, M. J., Leitgeb, R. A., Unterhuber, A., ... & Widhalm, G. (2019). Widefield fluorescence lifetime imaging of protoporphyrin IX for fluorescence‐guided neurosurgery: An ex vivo feasibility study. Journal of biophotonics, 12(6), e201800378.
Koren, K., Moßhammer, M., Scholz, V. V., Borisov, S. M., Holst, G., & Kühl, M. (2019). Luminescence Lifetime Imaging of Chemical Sensors—A Comparison between Time-Domain and Frequency-Domain Based Camera Systems. Analytical chemistry, 91(5), 3233-3238.
Moßhammer, M., Scholz, V. V., Holst, G., Kühl, M., & Koren, K. (2019). Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System. Journal of visualized experiments: JoVE, (154).
Wu, H. M., Lee, T. A., Ko, P. L., Liao, W. H., Hsieh, T. H., & Tung, Y. C. (2019). Widefield frequency domain fluorescence lifetime imaging microscopy (FD-FLIM) for accurate measurement of oxygen gradients within microfluidic devices. Analyst, 144(11), 3494-3504.