OASIS Implant, designed and produced by Mightex Systems, is a ground-breaking system that is capable of performing simultaneous all-optical recording (e.g. Calcium imaging) and manipulation (e.g. optogenetic stimulation) in the deep brain of a freely behaving animal to observe neural circuit activity with high cellular resolution.
Compatible with high-sensitivity research-grade cameras, OASIS Implant enables researchers to acquire high quality deep-brain images with low noise and high linearity, enabling quantitative data analysis. Equipped with both wide-field and patterned illumination capabilities (when combined with Mightex's market-leading Polygon400), OASIS Implant allows scientists to simultaneously perform Calcium imaging and targeted optogenetic stimulation with pin-point accuracy to stimulate individual neurons.
The super light-weight head mount offers many additional benefits. Since both deep-brain imaging and stimulation are delivered through a very thin imaging fiber, the overall weight of the head-mounted fixture is as little as 0.7g (compared to approximately 2~3g with a head-mounted camera), which minimizes stress experienced by the animal, and allows for the duration of the experiments to be extended. All of these features combined make the OASIS Implant an ideal research tool for in vivo experiments.
Superior Deep-Brain Imaging Capability
The OASIS Implant provides single-cell and population resolution imaging for a wide-range of experimental setups. With a standard c-mount camera port, OASIS Implant accepts any cameras, including research-grade scientific cameras which enable scientists to capture high-quality deep-brain images and videos. Furthermore, the OASIS Implant is compatible with multiple light sources to image different imaging indicators and fluorophores. Thus, the OASIS Implant will enable scientists to acquire more detailed and more accurate data for not only qualitative, but also quantitative analysis.
Deep Brain Wide-field and Targeted Optogenetic Stimulation
The Imaging Fiber Probe (IFP) consists of thousands of individual mini-fibers or pixels, and each of these pixels can be independently addressed with Miughtex's market-leading Polygon400 patterned illuminator. Therefore, the OASIS Implant can be used to deliver light to deep brain regions with sub-cellular resolution, and this opens the door for scientists to conduct experiments, such as deep-brain sub-cellular optogenetic stimulation or targeted calcium imaging, in a freely-moving animal. In addition, traditional wide-field illumination can easily be supported by sending light through all the individual pixels in the IFP. The OASIS Implant platform has two illumination ports, each capable of taking either a wide-field or a patterned illuminator with single or multiple wavelengths/colors.
The OASIS Implant enables imaging and stimulation in a freely-moving animal. This is due, in part, to the light-weight headmount (<0.7g) and the system being external from the animal. These requirements will help extend the length of experiments and reduce stress for imaging and stimulation experiments.